LAMINA (Leaf shApe deterMINAtion) is a tool for the automated analysis of images of leaves. LAMINA has been designed to provide classical measures of leaf shape (blade dimensions) and size (area) that are typically required for correlation analysis to biomass productivity, as well as measures that indicate asymmetry in leaf shape, leaf serration traits, and measures of herbivory damage (missing leaf area). The location of a chosen number of equally spaced markers can optionally be returned to support multivariate analysis, e.g. principal component analysis.